DNMT3B antibody (AA 251-350) (Biotin)

Catalog No. ABIN5675300

This anti-DNMT3B antibody is a Rabbit Polyclonal antibody detecting DNMT3B in WB. Suitable for Human and Mouse.

Quick Overview for DNMT3B antibody (AA 251-350) (Biotin) (ABIN5675300)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3B antibody is conjugated to Biotin

Application: Western Blotting (WB)

Product Details anti-DNMT3B Antibody (Biotin)

Binding Specificity: AA 251-350

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Dnmt3b

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: Dnmt3b

Background:

Synonyms: DNA (cytosine-5)-methyltransferase 3B, DNMT3B, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB

Background: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398).

Gene ID: 1789

UniProt: Q9UBC3