PUF60 antibody (AA 1-100) (Biotin)

Catalog No. ABIN5675527

The Rabbit Polyclonal anti-PUF60 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect PUF60 in samples from Human, Mouse and Rat.

Quick Overview for PUF60 antibody (AA 1-100) (Biotin) (ABIN5675527)

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUF60 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PUF60 Antibody (Biotin)

Binding Specificity: AA 1-100

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PUF60

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Alternative Name: PUF60

Background:

Synonyms: 60 kDa poly(U)-binding-splicing factor, FBP-interacting repressor, FIR, FLJ31379, FUSE-binding protein-interacting repressor, Poly U binding splicing factor 60KDa, Poly(U)-binding-splicing factor PUF60, poly-U binding splicing factor PUF60, PUF60, PUF60_HUMAN, pyrimidine tract binding splicing factor, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, RoBP1, RoBPI, siah binding protein 1, Siah-binding protein 1, Siah-BP1, SIAHBP1

Background: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA, binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

Gene ID: 22627

UniProt: Q9UHX1