CHST3 antibody (AA 21-120)

Catalog No. ABIN5675719

The Rabbit Polyclonal anti-CHST3 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) and ICC. It is suitable to detect CHST3 in samples from Human and Mouse.

Quick Overview for CHST3 antibody (AA 21-120) (ABIN5675719)

Target: CHST3 (Carbohydrate (Chondroitin 6) Sulfotransferase 3 (CHST3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-CHST3 Antibody

Binding Specificity: AA 21-120

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CHST3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CHST3

Target: CHST3 (Carbohydrate (Chondroitin 6) Sulfotransferase 3 (CHST3))

Alternative Name: CHST3

Background:

Synonyms: Carbohydrate sulfotransferase 3, CHST3, Chondroitin 6-O-sulfotransferase 1, C6ST-1, Chondroitin 6-sulfotransferase, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0, GST-0

Background: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.

Gene ID: 9469

UniProt: Q7LGC8

Pathways: Glycosaminoglycan Metabolic Process