BUB1B antibody

Catalog No. ABIN567606

The Mouse Monoclonal anti-BUB1B antibody (Clone 8G1) (ABIN567606) specifically detects BUB1B in WB, IF and IP. The antibody is reactive with Human samples.

Quick Overview for BUB1B antibody (ABIN567606)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 8G1

Product Details anti-BUB1B Antibody

Specificity: This antibody reacts with BUBR1 (121 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry.

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B

Background: Metaphase checkpoint controls sense abnormalities in chromosome alignment during mitosis and prevent progression to anaphase until proper alignment has been attained. A number of proteins, including Mitotic arrest deficiency protein 2 (MAD2), Budding uninhibited benzimidazole 1 (BUB1) and Budding uninhibited benzimidazole receptor 1 (BUBR1), have been implicated in the metaphase checkpoint control in mammalian cells. BUB1 and BUBR1 both localize to kinetochores during mitosis, suggesting that they play a role in delaying anaphase until all chromosomes achieve correct, bipolar attachment to the spindle. BUB1 and BUBR1 respond differently to spindle dynamics, they are part of a common complex during mitosis and BUB1 and BUBR1 may integrate different 'spindle assembly signals' into a single signal which can then be interpreted by downstream cell cycle regulators.Synonyms: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1

Gene ID: 701