FITC antibody

Catalog No. ABIN567764

The Mouse Monoclonal anti-FITC antibody is suitable to detect FITC. It has been validated for WB, IHC (fro), IF and EIA.

Quick Overview for FITC antibody (ABIN567764)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: NI 239

Product Details anti-FITC Antibody

Purification: Purified

Immunogen: Highly purified Fluorescein Isothiocyanate isomer 1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL sterile distilled water

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 without foreign proteins.

Preservative: Without preservative

Handling Advice: Avoid repeated thawing and freezing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC

Target Type: Chemical

Background: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate