GRAP2 antibody

Catalog No. ABIN567995

The Mouse Monoclonal anti-GRAP2 antibody is suitable to detect GRAP2 in samples from Human. It has been validated for WB, IP and FACS.

Quick Overview for GRAP2 antibody (ABIN567995)

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: UW40

Product Details anti-GRAP2 Antibody

Specificity: This antibody detects recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A affinity chromatography

Immunogen: GST-fusion human GRAP2/GADS protein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for GRAP2

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Alternative Name: GRAP2

Background: GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.Synonyms: Adapter protein GRID, GADS, GRB-2-like protein, GRB2-related adapter protein 2, GRB2L, GRBLG, GRBX, GRID, Grf-40, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, SH3-SH2-SH3 adapter Mona

Gene ID: 9402

NCBI Accession: NP_004801

UniProt: O75791

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway