S100B antibody

Catalog No. ABIN5680133

This anti-S100B antibody is a Mouse Monoclonal antibody detecting S100B in IHC (p). Suitable for Cow.

Quick Overview for S100B antibody (ABIN5680133)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100B antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SH-B1

Product Details anti-S100B Antibody

Specificity: S-100 beta, S-100 A (alpha/beta) and S-100 B (beta/beta), not reactive with S-100 A0 (alpha/alpha), Calmodulin, Parvalbumin and other Ca-binding proteins

Cross-Reactivity (Details): Cattle, human, cat, rabbit, rat, pig

Characteristics: The S-100 antibody has frequently been used for the detection of melanoic tumours. In brain tumours and metastases the antibody may be used for differentiation between glia derived tissue and tissues derived from other cell types. S-100 helps in differentiation of Melanoma and Schwannoma from other soft tissue tumours. It is also found in tumours of the salivary glands. S-100 is a homo- or heterodimer filament protein, composed of an alpha and a beta chain. In normal tissues it is found in glia and ependyma of the brain. In the periphery it is found in Schwann Cells, Melanocytes and in Langerhans Cells of the skin. In lymph nodes Interdigitating Cells are labelled. S-100 A0, which is exprimed e.g. by neurons, heart muscle and monocytes, will not be detected by this monoclonal antibody. S-100 ß, S-100 A (alpha/beta) and S-100 B (beta/beta), not reactive with S-100 A0 (alpha/alpha), Calmodulin, Parvalbumin and other Ca-binding proteins.

Purification: Antibody solution

Immunogen: Purified S-100beta protein from bovine brain

Isotype: IgG1

Application Details

Application Notes: Formaldehyde-fixed tissue should be pre-treated with 0.1 % pronase to give best results. Unmasking methods involving microwave or steam cooking are not recommended!,Skin, myelinated nerves,IHC(P),60 min at RT,(liquid conc.) Dilution 1:20 - 1:50 IHC(P)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: stabilizing phosphate buffer  pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 μL working solution / test)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S-100 beta

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins