Fibronectin antibody

Catalog No. ABIN5680161

This Rabbit Polyclonal antibody specifically detects Fibronectin in IF, ELISA, IHC (p) and RIA. It exhibits reactivity toward Cow.

Quick Overview for Fibronectin antibody (ABIN5680161)

Target: Fibronectin

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Product Details anti-Fibronectin Antibody

Specificity: Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution)

Cross-Reactivity (Details): Cattle, cross-reaction with dog

Characteristics: This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution).

Purification: Affinity purified

Immunogen: Purified fibronectin from bovine plasma

Isotype: IgG

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine skin or liver,IFA, IHC(P), ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:80, IHC(P) 1:500 (Envision), ELISA 1:200 (OD>500)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

Concentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

Preservative: Without preservative

Storage: -20 °C

Target Details for Fibronectin

Target: Fibronectin