ACSS2 antibody (Biotin)

Catalog No. ABIN568047

The Biotin-conjugated Rabbit Polyclonal anti-ACSS2 antibody (ABIN568047) specifically detects ACSS2 in WB, IF, IP, EIA, DB, ID and RIA. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for ACSS2 antibody (Biotin) (ABIN568047)

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSS2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-ACSS2 Antibody (Biotin)

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: ~5.5

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Purified S-Acetyl Coenzyme A Synthetase from baker's Yeast

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for ACSS2

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Alternative Name: ACSS2

Background: Acetyl Coenzyme A Synthetase is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. It acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene.Synonyms: ACAS2, ACSA, Acetate-CoA ligase, Acetyl-CoA synthetase, Acetyl-coenzyme A synthetase cytoplasmic, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme

Gene ID: 55902

NCBI Accession: NP_061147

UniProt: Q9NR19