ADA antibody (Biotin)

Catalog No. ABIN568053

This anti-ADA antibody is a Rabbit Polyclonal antibody detecting ADA in WB, IP, IF, EIA, DB, ID and RIA. Suitable for Cow.

Quick Overview for ADA antibody (Biotin) (ABIN568053)

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-ADA Antibody (Biotin)

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: ~5.4

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Adenosine Deaminase isolated and purified from Calf Intestine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Background: ADA (Adenosine deaminase) is an enzyme involved in the purine catabolic pathway. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Defects in ADA are a cause of autosomal recessive severe combined immuno-deficiency (SCID).Synonyms: Adenosine aminohydrolase

Gene ID: 280712

NCBI Accession: NP_776312

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process