Alanine Dehydrogenase antibody (Biotin)
Reactivity: Bacillus
DB, EIA, ID, IF, IP, RIA, WB
Host: Rabbit
Polyclonal
Biotin
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- Target See all Alanine Dehydrogenase products
- Alanine Dehydrogenase
- Reactivity
- Bacillus
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Alanine Dehydrogenase antibody is conjugated to Biotin
- Application
- Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
- Specificity
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes L-Alanine Dehydrogenase.
- Characteristics
- Molar Ratio: ~5.4
- Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
- Immunogen
- L-Alanine Dehydrogenase isolated and purified from Bacillus subtilis. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore by adding 1.0 mL of sterile distilled water.
- Concentration
- 10.0 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins.
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Target
- Alanine Dehydrogenase
- Abstract
- Alanine Dehydrogenase Products
- Background
- This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation.Synonyms: BSU31930, Stage V sporulation protein N, ald, spoVN
- Gene ID
- 936557
- NCBI Accession
- NP_391071
- UniProt
- Q08352
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