Alanine Dehydrogenase antibody (Biotin)
This anti-Alanine Dehydrogenase antibody is a Rabbit Polyclonal antibody detecting Alanine Dehydrogenase in DB, EIA, ID, IF, IP, RIA and WB. Suitable for Bacillus.
Catalog No. ABIN568056
Quick Overview for Alanine Dehydrogenase antibody (Biotin) (ABIN568056)
Target
Alanine Dehydrogenase
Reactivity
Bacillus
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Alanine Dehydrogenase antibody is conjugated to Biotin
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
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Specificity
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes L-Alanine Dehydrogenase.
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Characteristics
- Molar Ratio: ~5.4
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Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
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Immunogen
- L-Alanine Dehydrogenase isolated and purified from Bacillus subtilis. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
- IgG
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Restore by adding 1.0 mL of sterile distilled water.
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Concentration
- 10.0 mg/mL
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Buffer
- PBS, pH 7.2 without preservatives and foreign proteins.
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Preservative
- Without preservative
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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