L-Asparaginase 1 antibody (Biotin)

Catalog No. ABIN568114

Product Details

Target: L-Asparaginase 1

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunofluorescence (IF), Radioimmunoassay (RIA)

Specificity: The antibody recognizes L-Asparaginase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~5.5

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: L-Asparaginase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details

Target: L-Asparaginase 1

Background: Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L Asparaginase isoenzymes: L Asparaginase I, a low affinity enzyme located in the cytoplasm, and L Asparaginase II, a high affinity secreted enzyme.Synonyms: L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA

Gene ID: 946278

NCBI Accession: AP_002386

UniProt: P0A962