Catalase antibody (Biotin)

Catalog No. ABIN568133

The Rabbit Polyclonal anti-Catalase antibody has been validated for WB, IP, IF, EIA, RIA, ID and DB. It is suitable to detect Catalase in samples from Cow.

Quick Overview for Catalase antibody (Biotin) (ABIN568133)

Target: Catalase (CAT)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)

Product Details anti-Catalase Antibody (Biotin)

Specificity: The antibody recognizes catalase from Bovine Liver. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~5.4

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Catalase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: Catalase

Background: Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.Synonyms: CAT, Peroxisome Marker

Gene ID: 531682

NCBI Accession: NP_001030463

UniProt: P00432

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism