The Rabbit Polyclonal anti-Choline Oxidase antibody is suitable to detect Choline Oxidase in samples from Arthrobacter globiformis. It has been validated for WB, IP, EIA, IF, DB, ID and RIA.
Quick Overview for Choline Oxidase antibody (Biotin) (ABIN568151)
Target
Choline Oxidase
Reactivity
Arthrobacter globiformis
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Choline Oxidase antibody is conjugated to Biotin
Application
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
Characteristics
Molar Ratio: Biotin/IgG ~5.6
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Choline Oxidase isolated and purified from Athrobacter globiformis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Target
Choline Oxidase
Background
Choline Oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.