Butyrylcholinesterase antibody

Catalog No. ABIN568155

The Rabbit Polyclonal anti-Butyrylcholinesterase antibody (ABIN568155) specifically detects Butyrylcholinesterase in WB, IP, IF, EIA, DB, ID and RIA. The antibody is reactive with Human samples.

Quick Overview for Butyrylcholinesterase antibody (ABIN568155)

Target: Butyrylcholinesterase (BCHE)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Butyrylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-Butyrylcholinesterase Antibody

Specificity: The antibody recognizes Butyrylcholinesterase from Human Serum. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Solid Phase Affinity Chromatography

Immunogen: Butyrylcholinesterase isolated and purified from Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL of sterile distilled water, Prepare working dilutions by adding sterile phosphate buffered saline (PBS, ph 7.2) and preferably use the same day.

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C Reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Butyrylcholinesterase

Target: Butyrylcholinesterase (BCHE)

Alternative Name: Cholinesterase

Background: Butyrylcholinesterase is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. This enzyme is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits. Defects in Butyrylcholinesterase are the cause of a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics.Synonyms: Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, CHE1, Choline esterase II, Pseudocholinesterase

Gene ID: 590

NCBI Accession: NP_000046

UniProt: P06276

Pathways: Peptide Hormone Metabolism