ENO3 antibody (Biotin)

Catalog No. ABIN568187

The Sheep Polyclonal anti-ENO3 antibody has been validated for WB, IF, IP, ID, RIA, EIA and DB. It is suitable to detect ENO3 in samples from Rabbit.

Quick Overview for ENO3 antibody (Biotin) (ABIN568187)

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This ENO3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunodiffusion (ID), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA), Dot Blot (DB)

Product Details anti-ENO3 Antibody (Biotin)

Specificity: The antibody recognizes Enolase from Rabbit muscle The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~6.7

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Enolase isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for ENO3

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Alternative Name: Enolase beta

Background: Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO3, ENOB, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase

Gene ID: 100008769

NCBI Accession: NP_001075554

UniProt: P25704