ALT antibody (Biotin)

Catalog No. ABIN568223

The Rabbit Polyclonal anti-ALT antibody has been validated for WB, IP, IF, EIA, ID, DB and RIA. It is suitable to detect ALT in samples from Pig.

Quick Overview for ALT antibody (Biotin) (ABIN568223)

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALT antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)

Product Details anti-ALT Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG ~ 4.2

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Glutamic-Pyruvic Transaminase isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: Alanine Aminotransferase 1 (ALT1)

Background: Alanine Transaminase [glutamic pyruvate transaminase - GPT] participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Pyridoxal phosphate is a known cofactor. Expression is induced by glucocorticoids. This enzyme is present in a high concentration in liver tissue and it's release into the blood indicates liver disease or damage.Synonyms: AAT1, GPT, GPT1, Glutamate pyruvate transaminase 1, Glutamic-alanine transaminase 1, Glutamic-pyruvic transaminase 1

Gene ID: 9823

UniProt: P13191