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Glycerol Kinase antibody (Biotin)

The Rabbit Polyclonal anti-Glycerol Kinase antibody has been validated for WB, IF, IP, EIA, ID, RIA and DB. It is suitable to detect Glycerol Kinase in samples from Bacillus.
Catalog No. ABIN568238

Quick Overview for Glycerol Kinase antibody (Biotin) (ABIN568238)

Target

See all Glycerol Kinase (GK) Antibodies
Glycerol Kinase (GK)

Reactivity

  • 60
  • 21
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  • 7
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  • 6
  • 4
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  • 3
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  • 2
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Bacillus

Host

  • 72
  • 6
  • 4
Rabbit

Clonality

  • 80
  • 2
Polyclonal

Conjugate

  • 41
  • 9
  • 7
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Glycerol Kinase antibody is conjugated to Biotin

Application

  • 53
  • 41
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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
  • Characteristics

    Molar Ratio: Biotin/IgG ~4.6

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogen

    Glycerokinase isolated and purified from Bacillus stearothermophilus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Restore by adding 1.0 mL of sterile distilled water

    Concentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Glycerol Kinase (GK)

    Alternative Name

    Glycerol Kinase

    Background

    Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors, UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway.Synonyms: ATP:glycerol 3-phosphotransferase, GK, GKD, Glycerokinase

    Gene ID

    939741

    NCBI Accession

    NP_388810

    UniProt

    P18157
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