GUK1 antibody (Biotin)

Catalog No. ABIN568250

The Rabbit Polyclonal anti-GUK1 antibody has been validated for WB, IP, IF, DB, EIA, ID and RIA. It is suitable to detect GUK1 in samples from Pig.

Quick Overview for GUK1 antibody (Biotin) (ABIN568250)

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GUK1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-GUK1 Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG = ~6.6

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Guanylate kinase isolated and purified from Porcine brain. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for GUK1

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Alternative Name: Guanylate Kinase / GUK1

Background: Guanylate kinase catalyzes the transfer of phosphate from adenosine triphosphate (ATP) to guanosine monophosphate (GMP) or dGMP. This enzyme functions in the recovery of cGMP and is, therefore, thought to regulate the supply of guanine nucleotides to signal transduction pathways. The GUK2 and GUK3 isoforms are determined by separate loci. Brady et al. (1996) cloned human and mouse cDNAs of GUK1. They stated that the guanylate kinases are targets for cancer chemotherapy and are inhibited by the antitumor drug 6-thioguanine. They reported that the human gene codes for a protein of 197 amino acids with a mass of 21.7 kD. They found that the 1-kb message was ubiquitously expressed.Synonyms: GMK, GMP kinase

Gene ID: 9823

UniProt: P31006

Pathways: Nucleotide Phosphorylation, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process