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Pyrophosphatase (Inorganic) 1 (PPA1) antibody

The Rabbit Polyclonal anti- antibody has been validated for WB, IF, EIA, IP, RIA, DB and ID. It is suitable to detect in samples from Saccharomyces cerevisiae.
Catalog No. ABIN568268

Quick Overview for Pyrophosphatase (Inorganic) 1 (PPA1) antibody (ABIN568268)

Target

See all Pyrophosphatase (Inorganic) 1 (PPA1) Antibodies
Pyrophosphatase (Inorganic) 1 (PPA1)

Reactivity

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Saccharomyces cerevisiae

Host

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Rabbit

Clonality

  • 37
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Polyclonal

Conjugate

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Un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB), Immunodiffusion (ID)
  • Specificity

    Inorganic Pyrophosphatase is isolated and purified from Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogen

    Inorganic Pyrophosphatase is isolated and purified from Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 1.0 mL sterile distilled water

    Concentration

    10 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Pyrophosphatase (Inorganic) 1 (PPA1)

    Alternative Name

    Pyrophosphatase 1 / PPA1

    Background

    Pyrophosphatase 1 (PPA1) is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells.Synonyms: IOPPP, Inorganic pyrophosphatase, PP, PPase, Pyrophosphate phospho-hydrolase

    Gene ID

    852296

    NCBI Accession

    NP_009565

    UniProt

    P00817
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