Lactate Dehydrogenase (4H) (LDH-1) antibody (Biotin)

Catalog No. ABIN568284

The Biotin-conjugated Rabbit Polyclonal anti-Lactate Dehydrogenase (4H) antibody (ABIN568284) specifically detects Lactate Dehydrogenase (4H) in WB, IF, DB, EIA, ID, IP and RIA. The antibody is reactive with Human samples.

Quick Overview for Lactate Dehydrogenase (4H) (LDH-1) antibody (Biotin) (ABIN568284)

Target: Lactate Dehydrogenase (4H) (LDH-1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Product Details

Characteristics: Molar Ratio: Biotin/IgG = ~6.0

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: L-Lactic dehydrogenase, LDH-1(H4) isoenzyme isolated and purified from Human erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details

Target: Lactate Dehydrogenase (4H) (LDH-1)

Alternative Name: LDH1 H4 Isozyme

Background: Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.Synonyms: L-Lactic dehydrogenase LDH-1 Isozyme H4