LDH5 antibody (Biotin)

Catalog No. ABIN568301

The Sheep Polyclonal anti-LDH5 antibody has been validated for WB, IF, EIA, DB, ID, IP and RIA. It is suitable to detect LDH5 in samples from Rabbit.

Quick Overview for LDH5 antibody (Biotin) (ABIN568301)

Target: LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This LDH5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Product Details anti-LDH5 Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG ~ 6.7

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: L-Lactic Dehydrogenase LDH5(M4) isoenzyme, isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for LDH5

Target: LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))

Background: Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: L-Lactic dehydrogenase LDH-5 Isozyme M4