Luciferase antibody (Biotin)

Catalog No. ABIN568305

This anti-Luciferase antibody is a Rabbit Polyclonal antibody detecting Luciferase in WB, IF, IP, DB, EIA, RIA and ID. Suitable for Photinus pyralis.

Quick Overview for Luciferase antibody (Biotin) (ABIN568305)

Target: Luciferase

Reactivity: Photinus pyralis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Luciferase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Dot Blot (DB), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID)

Product Details anti-Luciferase Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG: ~1.7

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Luciferase isolated and purified from Photinus pyralis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Luciferase

Target: Luciferase

Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.Synonyms: Luciferin 4-monooxygenase

Gene ID: 7054

UniProt: P08659