PGK1 antibody (Biotin)

Catalog No. ABIN568372

This Rabbit Polyclonal antibody specifically detects PGK1 in WB, IF, IP, EIA, DB, ID and RIA. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for PGK1 antibody (Biotin) (ABIN568372)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-PGK1 Antibody (Biotin)

Specificity: 3-Phosphoglyceric Phosphokinase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~6.1

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: 3-Phosphoglyceric phosphokinase isolated and purified from baker's yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1

Background: Synonyms: Phosphoglycerate kinase, YCR012W, YCR12W

Gene ID: 850370

NCBI Accession: NP_009938

UniProt: P00560

Pathways: Cellular Glucan Metabolic Process