The Sheep Polyclonal anti-PHKA1 antibody has been validated for WB, IF, DB, EIA, ID, IP and RIA. It is suitable to detect PHKA1 in samples from Rabbit.
IgG fraction of polyclonal sheep antiserum to phosphorylase a from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Phosphorylase a isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.