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PHKB antibody (Biotin)

This anti-PHKB antibody is a Sheep Polyclonal antibody detecting PHKB in WB, IP, IF, DB, EIA, ID and RIA. Suitable for Rabbit.
Catalog No. ABIN568401

Quick Overview for PHKB antibody (Biotin) (ABIN568401)

Target

See all PHKB Antibodies
PHKB (Phosphorylase Kinase, beta (PHKB))

Reactivity

  • 36
  • 9
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Rabbit

Host

  • 35
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Sheep

Clonality

  • 37
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Polyclonal

Conjugate

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  • 1
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  • 1
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This PHKB antibody is conjugated to Biotin

Application

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Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA)
  • Specificity

    Biotin-conjugated IgG fraction of polyclonal sheep antiserum to phosphorylase b from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

    Characteristics

    Molar Ratio: Biotin/IgG ~5.8

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogen

    Phosphorylase b isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Restore with 1,0 mL distilled water

    Concentration

    10,0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Preservative

    Without preservative

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
  • Target

    PHKB (Phosphorylase Kinase, beta (PHKB))

    Alternative Name

    PHKB

    Background

    Synonyms: Phosphorylase b kinase regulatory subunit beta, Phosphorylase kinase subunit beta

    Gene ID

    100009137

    NCBI Accession

    NP_001075770

    UniProt

    P12798

    Pathways

    Cellular Glucan Metabolic Process
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