cFBPase (Cytosolic) antibody

Catalog No. ABIN5684015

This Rabbit Polyclonal antibody specifically detects cFBPase in IL and WB. It exhibits reactivity toward Arabidopsis thaliana, Macroptilium atropurpureum, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Pinus sylvestris, Pinus yunnanensis, Potato, Rape and Zea mays. It has been mentioned in 7+ publications

Quick Overview for cFBPase (Cytosolic) antibody (ABIN5684015)

Target: cFBPase

Reactivity: Arabidopsis thaliana, Macroptilium atropurpureum, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Pinus sylvestris, Pinus yunnanensis, Potato, Rape, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Product Details

Binding Specificity: Cytosolic

Purpose: Anti-cFBPase | Cytosolic fructose-1,6-bisphosphatase (cytoplasm marker in photosynthetic tissues)

Cross-Reactivity (Details): Arabidopsis thaliana, Brassica napus, Macroptilium atropurpureum, Nicotiana benthamiana, Pinus silvestris, Pinus yunanniensis, Oryza sativa, Petunia hybrida cv. Mitchell, Solanum tuberosum, Zea mays
Not reactive in: Chlamydomonas reinhardtii

Predicted Reactivity: Capsella rubella,Pisum sativum,Ricinus communis,Glycine max,Phaseolus vulgaris,Sesamum indicum,Spinacia oleracea,Populus trichocarpa,Vitis vinifera

Purification: Serum

Immunogen: Overexpressed cytosolic fructose 1

Application Details

Application Notes: 1: 500 (IL), 1 : 5 000 (WB)

Comment:

for image of detection please refer to Strand et al. 2000. This antibody does not react with chloroplastic form of FBPase.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Liu, Fan, Li, Song, Li, Zhang, Xue: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).

Iglesias, Trigueros, Rojas-Triana, Fernández, Albar, Bustos, Paz-Ares, Rubio: "Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodeling in the Arabidopsis response to phosphate starvation." in: Journal of proteomics, Vol. 94C, pp. 1-22, (2013) (PubMed).

Rautengarten, Ebert, Ouellet, Nafisi, Baidoo, Benke, Stranne, Mukhopadhyay, Keasling, Sakuragi, Scheller: "Arabidopsis Deficient in Cutin Ferulate encodes a transferase required for feruloylation of ω-hydroxy fatty acids in cutin polyester." in: Plant physiology, Vol. 158, Issue 2, pp. 654-65, (2012) (PubMed).

Hayashi, Wakasa, Takahashi, Kawakatsu, Takaiwa: "Signal transduction by IRE1-mediated splicing of bZIP50 and other stress sensors in the endoplasmic reticulum stress response of rice." in: The Plant journal : for cell and molecular biology, Vol. 69, Issue 6, pp. 946-56, (2012) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

Han, Chen, Dai, Yang, Wang: "Isobaric tags for relative and absolute quantification- based comparative proteomics reveals the features of plasma membrane-associated proteomes of pollen grains and pollen tubes from Lilium davidii." in: Journal of integrative plant biology, Vol. 52, Issue 12, pp. 1043-58, (2010) (PubMed).

Strand, Zrenner, Trevanion, Stitt, Gustafsson, Gardeström: "Decreased expression of two key enzymes in the sucrose biosynthesis pathway, cytosolic fructose-1,6-bisphosphatase and sucrose phosphate synthase, has remarkably different consequences for photosynthetic carbon metabolism in transgenic Arabidopsis thalian" in: The Plant journal : for cell and molecular biology, Vol. 23, Issue 6, pp. 759-70, (2000) (PubMed).

Target Details

Target: cFBPase

Background:

Background: Fructose-1,6 bisphosphatase (FBPase) (EC=3.1.311) is one of the regulatory enzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1,6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours. Alternative name: D-fructose-1,6-bisphosphate 1-phosphohydrolase

Additional information: Kinetic and allosteric properties of the plant cytosolic FBPase are remarkably similar to the mammalian and yeast FBPase, but differ greatly from those of the chloroplastic FBPase. The antibody could detect FBPase from the human COS-7 cell line transfected with FBP1 expressing vector.This product can be sold containing ProClin if requested.

Molecular Weight: 45 | 37 kDa (Arabidopsis thaliana)

Gene ID: 840953

UniProt: Q9MA79