IdnK antibody (Biotin)

Catalog No. ABIN568460

The Rabbit Polyclonal anti-IdnK antibody has been validated for WB, IF, EIA, RIA, DB, ID and IP. It is suitable to detect IdnK in samples from E. coli.

Quick Overview for IdnK antibody (Biotin) (ABIN568460)

Target: IdnK (IDNK) (IdnK Gluconokinase Homolog (IDNK))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IdnK antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP)

Product Details anti-IdnK Antibody (Biotin)

Specificity: This antibody is polyclonal immunologic reagents to Gluconate kinase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~ 3.3

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Gluconate kinase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for IdnK

Target: IdnK (IDNK) (IdnK Gluconokinase Homolog (IDNK))

Alternative Name: Gluconate Kinase

Background: Synonyms: Gluconokinase, Thermosensitive gluconokinase, gntV, idnK

Gene ID: 946066

NCBI Accession: AP_004764

UniProt: P39208