Thermonuclease antibody (Biotin)

Catalog No. ABIN568479

The Biotin-conjugated Rabbit Polyclonal anti-Thermonuclease antibody (ABIN568479) specifically detects Thermonuclease in DB, EIA, ID, IF, IP, RIA and WB. The antibody is reactive with Staphylococcus aureus samples.

Quick Overview for Thermonuclease antibody (Biotin) (ABIN568479)

Target: Thermonuclease

Reactivity: Staphylococcus aureus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)

Product Details

Characteristics: Molar Ratio: Biotin/IgG ~4.8

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Nuclease isolated and purified from Staphylococcus aureus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/m

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details

Target: Thermonuclease

Background: Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3'-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems.Synonyms: Nuclease, TNase, nuc

Gene ID: 1280

UniProt: P00644