DEFB4A antibody (Biotin)

Catalog No. ABIN5687571

This Goat Polyclonal antibody specifically detects DEFB4A in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for DEFB4A antibody (Biotin) (ABIN5687571)

Target: DEFB4A (DEFB4) (Defensin, beta 4A (DEFB4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DEFB4A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-DEFB4A Antibody (Biotin)

Purification: Anti-hBD-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBD-2 (human BD-2).

Application Details

Application Notes: ELISA:

To detect hBD-2 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hBD-2.

Western Blot:
To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: BD-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for DEFB4A

Target: DEFB4A (DEFB4) (Defensin, beta 4A (DEFB4))

Alternative Name: BD-2

Gene ID: 1673

UniProt: O15263