FASL antibody

Catalog No. ABIN5687580

This Goat Polyclonal antibody specifically detects FASL in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for FASL antibody (ABIN5687580)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-FASL Antibody

Purification: Anti-hFasL specific antibody was purified by affinity chromatography employing immobilized hFasL matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL (human FAS Ligand).

Application Details

Application Notes: Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of FasL/Apo1L (10 ng/mL), a concentration of 0.08 - 0.1 μ,g/mL of this antibody is required. ELISA:

To detect hFasL by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFasL.

Western Blot:
To detect hFasL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Fas Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: Fas Ligand

Background: Fas Ligand is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.

Gene ID: 356

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity