FGF16 antibody (Biotin)

Catalog No. ABIN5687584

The Goat Polyclonal anti-FGF16 antibody is suitable to detect FGF16 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for FGF16 antibody (Biotin) (ABIN5687584)

Target: FGF16 (Fibroblast Growth Factor 16 (FGF16))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGF16 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-FGF16 Antibody (Biotin)

Purification: Anti-hFGF-16 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFGF-16 (human FGF-16).

Application Details

Application Notes: ELISA:

To detect hFGF-16 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFGF-16.

Sandwich: To detect hFGF-16 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-16 (XP-5132) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-16.

Western Blot:
To detect hFGF-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: FGF-16 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for FGF16

Target: FGF16 (Fibroblast Growth Factor 16 (FGF16))

Alternative Name: FGF-16

Gene ID: 8823

UniProt: O43320

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway