IL-17 antibody

Catalog No. ABIN5687587

The Goat Polyclonal anti-IL-17 antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL-17 in samples from Human.

Quick Overview for IL-17 antibody (ABIN5687587)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-17 Antibody

Purification: Anti-hIL-17 specific antibody was purified by affinity chromatography employing immobilized hIL-17 matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-17 (human Interleukin-17).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-17A (50.00 ng/mL), a concentration of 0.43 - 0.65 μ,g/mL of this antibody is required.

ELISA:

To detect hIL-17A by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.

Sandwich:

To detect hIL-17A by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-17A (XP-5171Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.

Western Blot:

To detect hIL-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-17 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-17

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Alternative Name: IL-17

Gene ID: 3605

UniProt: Q16552