CXCL7 antibody (Biotin)

Catalog No. ABIN5687597

The Biotin-conjugated Goat Polyclonal anti-CXCL7 antibody (ABIN5687597) specifically detects CXCL7 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CXCL7 antibody (Biotin) (ABIN5687597)

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCL7 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CXCL7 Antibody (Biotin)

Purification: Anti-hNAP-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNAP-2 (human NAP-2).

Application Details

Application Notes: ELISA:

To detect hNAP-2 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hNAP-2.

Sandwich:

To detect hNAP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human NAP-2 (XP-5245) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2.

Western Blot:
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: NAP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL7

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Alternative Name: NAP-2

Background: NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Gene ID: 5473

UniProt: P02775