Neuropilin 1 antibody (Biotin)

Catalog No. ABIN5687599

The Goat Polyclonal anti-Neuropilin 1 antibody has been validated for WB and ELISA. It is suitable to detect Neuropilin 1 in samples from Human.

Quick Overview for Neuropilin 1 antibody (Biotin) (ABIN5687599)

Target: Neuropilin 1 (NRP1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neuropilin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Neuropilin 1 Antibody (Biotin)

Purification: Anti-hNP-1 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNP-1 (Human NP-1).

Application Details

Application Notes: ELISA:

To detect hNP-1 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hNP-1.

Sandwich:

To detect hNP-1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human NP-1 (XP-5247) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNP-1.

Western Blot:

To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: NP-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Neuropilin 1

Target: Neuropilin 1 (NRP1)

Alternative Name: NP-1

Gene ID: 1667

UniProt: P59665

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals