Neurotrophin 3 antibody

Catalog No. ABIN5687601

This Goat Polyclonal antibody specifically detects Neurotrophin 3 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for Neurotrophin 3 antibody (ABIN5687601)

Target: Neurotrophin 3 (NTF3)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neurotrophin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Neurotrophin 3 Antibody

Purification: Anti-hNT 3 specific antibody was purified by affinity chromatography employing immobilized hNT-3 matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNT-3 (human Neurotrophin-3).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hNT-3 (50.00 ng/mL), a concentration of 2.0 - 4.0 μ,g/mL of this antibody is required.

ELISA:

To detect hNT-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNT-3.

Sandwich:

To detect hNT-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NT-3 (XP-5249Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3.

Western Blot:
To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: NT-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: NT-3

Gene ID: 4908

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway