CD40 Ligand antibody (Biotin)

Catalog No. ABIN5687607

This Goat Polyclonal antibody specifically detects CD40 Ligand in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD40 Ligand antibody (Biotin) (ABIN5687607)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CD40 Ligand Antibody (Biotin)

Purification: Anti-hsCD40L specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L (Human sCD40L).

Application Details

Application Notes: ELISA:

To detect hsCD40L by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hsCD40L.

Sandwich:

To detect hsCD40L by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sCD40L (XP-5263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L.

Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: sCD40 Ligand

Gene ID: 959

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints