EGF antibody (Biotin)

Catalog No. ABIN5687626

The Goat Polyclonal anti-EGF antibody has been validated for WB, ELISA and Neut. It is suitable to detect EGF in samples from Mouse.

Quick Overview for EGF antibody (Biotin) (ABIN5687626)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This EGF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-EGF Antibody (Biotin)

Purification: Anti-mEGF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEGF (murine EGF).

Application Details

Application Notes: Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Murine EGF (0.1 ng/mL), a concentration of 3.0-5.0 ng/mL of this antibody is required.ELISA:

To detect mEGF by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mEGF.

Western Blot:
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: EGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Gene ID: 13645

UniProt: P01132

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation