IL18BP antibody (Biotin)

Catalog No. ABIN5687629

The Goat Polyclonal anti-IL18BP antibody is suitable to detect IL18BP in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for IL18BP antibody (Biotin) (ABIN5687629)

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL18BP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL18BP Antibody (Biotin)

Purification: Anti-mIL-18 BP specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-18 BP (murine Interleukin-18 Binding Protein).

Application Details

Application Notes: ELISA:

To detect mIL-18 BP by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-18 BP.

Sandwich

To detect mIL-18BP by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-18BP (XP-5173) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-18BP.

Western Blot:

To detect mIL-18 BP by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-18 BP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-18 Binding Protein antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL18BP

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Alternative Name: IL-18 Binding Protein

Gene ID: 16068

UniProt: Q9Z0M9