VEGF antibody

Catalog No. ABIN5688712

The Mouse Monoclonal anti-VEGF antibody has been validated for WB, ELISA and Neut. It is suitable to detect VEGF in samples from Human.

Quick Overview for VEGF antibody (ABIN5688712)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 4B2-8

Product Details anti-VEGF Antibody

Purification: This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.

Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of <0.2 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μ,g/mL.

Western Blot:

To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF

Gene ID: 7422

UniProt: P15692