CCL25 antibody

Catalog No. ABIN5688717

This Mouse Monoclonal antibody specifically detects CCL25 in ELISA, WB and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL25 antibody (ABIN5688717)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL25 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Clone: 1-2_4G1-1G4-1C9

Product Details anti-CCL25 Antibody

Purification: This IgG1k antibody was purified from ascites fluid on a Protein A affinity column.

Immunogen: The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTECK as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0-20.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 300 pg/mL of recombinant human TECK when used with our biotinylated antigen affinity purified anti-human TECK (XP-5280Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 0.50-1.0 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: TECK lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: TECK

Background: This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.

Gene ID: 6370

UniProt: O15444