CCL20 antibody

Catalog No. ABIN5688724

The Mouse Monoclonal anti-CCL20 antibody has been validated for WB, ELISA and Neut. It is suitable to detect CCL20 in samples from Human.

Quick Overview for CCL20 antibody (ABIN5688724)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: G3_4G10-3

Product Details anti-CCL20 Antibody

Purification: This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: MIP-3 alpha antibody is produced in mice using highly pure (>98%) recombinant human MIP- 3alpha as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 4.0-8.0 μ,g/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with our biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western blot:

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: MIP-3 alpha lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: MIP-3 alpha

Background: Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It binds to CCR6.

Was product number XP-5713-M

Gene ID: 6364

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection