CCL26 antibody

Catalog No. ABIN5688730

The Mouse Monoclonal anti-CCL26 antibody has been validated for WB, ELISA and Neut. It is suitable to detect CCL26 in samples from Human.

Quick Overview for CCL26 antibody (ABIN5688730)

Target: CCL26 (Chemokine (C-C Motif) Ligand 26 (CCL26))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL26 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 1-1_3H10-1D11

Product Details anti-CCL26 Antibody

Purification: This IgG2aK antibody was purified from ascites fluid by Protein A affinity chromatography.

Immunogen: Eotaxin-3 antibody is produced in BALB/c ICRR F1 mice using highly pure (>98%) recombinant human Eotaxin-3 as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL), a concentration of 1.0-5.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μ,g/mL.

Western Blot:
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: Eotaxin-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for CCL26

Target: CCL26 (Chemokine (C-C Motif) Ligand 26 (CCL26))

Alternative Name: Eotaxin-3

Gene ID: 10344

UniProt: Q9Y258

Pathways: Regulation of Actin Filament Polymerization