14-3-3 theta antibody (C-Term)

Catalog No. ABIN5688864

This Rabbit Polyclonal antibody specifically detects 14-3-3 theta in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Chicken, Dog, Xenopus laevis and Zebrafish (Danio rerio).

Quick Overview for 14-3-3 theta antibody (C-Term) (ABIN5688864)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-14-3-3 theta Antibody

Binding Specificity: C-Term

Purification: Affinity Purified

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).

Application Details

Application Notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: 14-3-3 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3

Background: 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.

Molecular Weight: 29 kDa

Gene ID: 56011

UniProt: P35213

Pathways: Apoptosis, Myometrial Relaxation and Contraction