CAMK2A antibody (pThr286)

Catalog No. ABIN5688927

This Rabbit Polyclonal antibody specifically detects CAMK2A in WB. It exhibits reactivity toward Human, Mouse, Rat and Xenopus laevis.

Quick Overview for CAMK2A antibody (pThr286) (ABIN5688927)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAMK2A Antibody

Binding Specificity: pThr286

Specificity: CaM Kinase II antibody is specific for a 50 kDa and b 60 kDa CaM Kinase II phosphorylated at Thr286.

Purification: Affinity Purified

Immunogen: CaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.

Application Details

Application Notes: Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CaM Kinase II

Background: Calcium/calmodulin kinase II (CaM Kinase II) is multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.

Molecular Weight: 50/60 kDa

Gene ID: 25400

UniProt: P11275

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction