CXCL11 antibody

Catalog No. ABIN5689018

The Rabbit Polyclonal anti-CXCL11 antibody has been validated for WB, ELISA and Neut. It is suitable to detect CXCL11 in samples from Human.

Quick Overview for CXCL11 antibody (ABIN5689018)

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL11 Antibody

Purification: Anti-hI-TAC specific antibody was purified by affinity chromatography employing immobilized hI-TAC matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hI-TAC (human Interferon-Inducible T Cell Alpha Chemoattractant).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hI-TAC (100 ng/mL), a concentration of 2.0 - 3.0 μ,g/mL of this antibody is required.

ELISA:

To detect hI-TAC by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hI-TAC.

Sandwich:

To detect hI-TAC by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human I-TAC (XP-5205Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-TAC.

Western Blot:

To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: I-TAC antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL11

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Alternative Name: I-TAC

Gene ID: 6373

UniProt: O14625