IGF2 antibody

Catalog No. ABIN5689024

The Rabbit Polyclonal anti-IGF2 antibody has been validated for WB, ELISA and Neut. It is suitable to detect IGF2 in samples from Human.

Quick Overview for IGF2 antibody (ABIN5689024)

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IGF2 Antibody

Purification: Anti-hIGF-II specific antibody was purified by affinity chromatography employing immobilized hIGF-II matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-II (human Insulin Like Growth Factor-II).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1 4.6 mg/mL of this antibody is required.

ELISA:

To detect hIGF-II by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-II.

Sandwich:

To detect hIGF-II by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.

Western Blot:

To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IGF-II antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IGF2

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Alternative Name: IGF-II

Gene ID: 3481

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation