IL16 antibody

Catalog No. ABIN5689034

The Rabbit Polyclonal anti-IL16 antibody (ABIN5689034) specifically detects IL16 in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for IL16 antibody (ABIN5689034)

Target: IL16 (Interleukin 16 (IL16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL16 Antibody

Purification: Anti-hIL-16 specific antibody was purified by affinity chromatography employing immobilized hIL-16 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-16 (human Interleukin-16).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.20 ng/mL), a concentration of 0.12 - 0.15 μ,g/mL of this antibody is required.

ELISA:

To detect hIL-16 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16.

Sandwich:

To detect hIL-16 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16.

Western Blot:

To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-16 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL16

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: IL-16

Background: IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.

Gene ID: 3603

UniProt: Q9UME6