IL-17 antibody (Biotin)

Catalog No. ABIN5689035

This Rabbit Polyclonal antibody specifically detects IL-17 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL-17 antibody (Biotin) (ABIN5689035)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-17 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL-17 Antibody (Biotin)

Purification: Anti-hIL-17 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17 (human Interleukin-17).

Application Details

Application Notes: ELISA:

To detect hIL-17 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-17.

Sandwich:

To detect hIL-17A by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-17A (XP-5171Bt) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.

Western Blot:

To detect hIL-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-17 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-17

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Alternative Name: IL-17

Gene ID: 3605

UniProt: Q16552