CXCL9 antibody

Catalog No. ABIN5689079

This anti-CXCL9 antibody is a Rabbit Polyclonal antibody detecting CXCL9 in WB, ELISA and Neut. Suitable for Human.

Quick Overview for CXCL9 antibody (ABIN5689079)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL9 Antibody

Purification: Anti-hMIG specific antibody was purified by affinity chromatography employing immobilized hMIG matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIG (human MIG).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/mL), a concentration of 5.0 - 7.0 μ,g/mL of this antibody is required.

ELISA:

To detect hMIG by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIG.

Sandwich:

To detect hMIG by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIG (XP-5231Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.

Western Blot:

To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIG antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: MIG

Background: MIG, a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor.

Gene ID: 4283

UniProt: Q07325